Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p109 | (1) | WCRB2014

Postovulatory ageing influences methylation marks and development in bovine oocytes and early embryos

Heinzmann Julia , Mattern Felix , Aldag Patrick , Lucas-Hahn Andrea , Wrenzycki Christine , Haaf Thomas , Niemann Heiner

Introduction: Oocytes that are not fertilized in a specific time-frame after ovulation undergo a time-dependent process of degradation and loss of developmental potential that can be referred to as postovulatory ageing. This process occurs in vivo and under specific in vitro settings. Here, we studied epigenetic changes related to postovulatory ageing.Materials and methods: Bovine oocytes were collected from slaughterhouse ovaries and m...

ra0001p050 | (1) | WCRB2014

Fingerprints on oocyte and embryo lipid profiles caused by in vitro embryo production system and fatty acid diet supplementation in bovine

Gonzalez Serrano Andres Felipe , Ferreira Christina R , Pirro Valentina , Lucas-Hahn Andrea , Heinzmann Julia , Hadeler Klaus-Gerd , Aldag Patrick , Baulain Ulrich , Piechotta Marion , Danicke Sven , Cooks Robert Graham , Niemann Heiner

Introduction: Lipid content of oocytes and early embryos is critical for embryonic and fetal development. Indeed, the success of assisted reproductive techniques (ARTs) such as cryopreservation and in vitro embryo production is heavily affected by the lipid content of preimplantation embryos.Materials and methods: Lipid profiling of single in vitro (IVTB) and in vivo (IVVB) produced bovine embryos was performed by desorption el...